Show simple item record

dc.contributor.authorTunsjø, Hege
dc.contributor.authorUllmann, Ingvild Falkum
dc.contributor.authorCharnock, Colin
dc.description.abstractAn important challenge relating to clinical diagnostics of the foodborne pathogen Shiga toxin-producing E. coli (STEC), is that PCR-detection of the shiga-toxin gene (stx) in DNA from stool samples can be accompanied by a failure to identify an STEC isolate in pure culture on agar. In this study, we have explored the use of MinION long-read sequencing of DNA from bacterial culture swipes to detect the presence of STEC, and bioinformatic tools to characterize the STEC virulence factors. The online workflow “What’s in my pot” (WIMP) in the Epi2me cloud service, rapidly identified STEC also when it was present in culture swipes together with multiple other E. coli serovars, given sufficient abundance. These preliminary results provide useful information about the sensitivity of the method, which has potential to be used in clinical diagnostic of STEC, particularly in cases where a pure culture of the STEC isolate is not obtained due to the ‘STEC lost Shiga toxin’ phenomenon.en_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.titleA preliminary study of the use of MinION sequencing to specifcally detect Shiga toxin‑producing Escherichia coli in culture swipes containing multiple serovars of this speciesen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.source.journalScientific Reportsen_US

Files in this item


This item appears in the following Collection(s)

Show simple item record

Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal