Show simple item record

dc.contributor.authorShwe, Alice
dc.contributor.authorKrasnov, Aleksei
dc.contributor.authorVisnovska, Martina
dc.contributor.authorRamberg, Sigmund
dc.contributor.authorTone-Kari, Østbye
dc.contributor.authorAndreassen, Rune
dc.description.abstractSmoltification is a developmental process that preadapts Atlantic salmon for a life in seawater. Suboptimal smoltification and poor timing of transfer to seawater is associated with increased mortality. MicroRNAs (miRNAs) are small non-coding genes. They regulate gene expression post-transcriptionally as part of the miRNA induce silencing complex (miRISC) where they guide miRISC to particular mRNAs (target genes). The aim of this study was to identify Atlantic salmon miRNAs expressed in liver that are associated with smoltification and adaptation to seawater as well as to predict their target genes. In total, 62 guide miRNAs were identified, and by their expression patterns they were clustered into three groups. Target gene predictions followed by gene enrichment analysis of the predicted targets indicated that the guide miRNAs were involved in post-transcriptional regulation of important smoltification associated biological processes. Some of these were energy metabolism, protein metabolism and transport, circadian rhythm, stress and immune response. Together, the results indicate that certain miRNAs are involved in the regulation of many of the important changes occurring in the liver during this developmental transition. Optimal smoltification is crucial for normal development, growth, and health of farmed Atlantic salmon in seawater. Here, we characterize miRNA expression in liver to reveal whether miRNAs regulate gene expression during this developmental transition. Expression changes of miRNAs and mRNAs was studied by small-RNA sequencing and microarray analysis, respectively. This revealed 62 differentially expressed guide miRNAs (gDE-miRNAs) that could be divided into three groups with characteristic dynamic expression patterns. Three of miRNA families are known as highly expressed in liver. A rare arm shift was observed during smoltification in the Atlantic salmon-specific novel-ssa-miR-16. The gDE-miRNAs were predicted to target 2804 of the genes revealing expression changes in the microarray analysis. Enrichment analysis revealed that targets were significantly enriched in smoltification-associated biological process groups. These included lipid and cholesterol synthesis, carbohydrate metabolism, protein metabolism and protein transport, immune system genes, circadian rhythm and stress response. The results indicate that gDE-miRNAs may regulate many of the changes associated with this developmental transition in liver. The results pave the way for validation of the predicted target genes and further study of gDE-miRNA and their targets by functional assays.en_US
dc.description.sponsorshipThis research was funded by THE RESEARCH COUNCIL OF NORWAY, grant number 280839/E40.en_US
dc.relation.ispartofseriesBiology (Basel);Volume 11 / Issue 5
dc.relation.uri biology11050688
dc.rightsNavngivelse 4.0 Internasjonal*
dc.subjectSeawater adaptationen_US
dc.subjectSmall-RNA sequencingen_US
dc.subjectAtlantic salmonen_US
dc.titleExpression Analysis in Atlantic Salmon Liver Reveals miRNAs Associated with Smoltification and Seawater Adaptationen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.rights.holder© 2022 by the authorsen_US
dc.source.journalBiology (Basel)en_US
dc.relation.projectNorges forskningsråd: 280839en_US
dc.subject.nsiVDP::Genetikk og genomikk: 474en_US
dc.subject.nsiVDP::Genetics and genomics: 474en_US
dc.subject.nsiVDP::Genetikk og genomikk: 474en_US
dc.subject.nsiVDP::Genetics and genomics: 474en_US

Files in this item


This item appears in the following Collection(s)

Show simple item record

Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal