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dc.contributor.authorTune, Benedicte Grebstad
dc.contributor.authorMelheim, Maria
dc.contributor.authorAtneosen-Åsegg, Monica
dc.contributor.authorDotinga, Baukje M.
dc.contributor.authorSaugstad, Ola Didrik
dc.contributor.authorSolberg, Rønnaug
dc.contributor.authorBaumbusch, Lars Oliver
dc.date.accessioned2024-01-26T12:02:53Z
dc.date.available2024-01-26T12:02:53Z
dc.date.created2023-04-18T13:30:04Z
dc.date.issued2023
dc.identifier.citationBiology (Basel). 2023, 12 (4), .en_US
dc.identifier.issn2079-7737
dc.identifier.urihttps://hdl.handle.net/11250/3114078
dc.description.abstractBirth asphyxia is the leading cause of death and disability in young children worldwide. Long non-coding RNAs (lncRNAs) may provide novel targets and intervention strategies due to their regulatory potential, as demonstrated in various diseases and conditions. We investigated cardinal lncRNAs involved in oxidative stress, hypoxia, apoptosis, and DNA damage using a piglet model of perinatal asphyxia. A total of 42 newborn piglets were randomized into 4 study arms: (1) hypoxia –normoxic reoxygenation, (2) hypoxia–3 min of hyperoxic reoxygenation, (3) hypoxia–30 min of hyperoxic reoxygenation, and (4) sham-operated controls. The expression of lncRNAs BDNF-AS, H19, MALAT1, ANRIL, TUG1, and PANDA, together with the related target genes VEGFA, BDNF, TP53, HIF1α, and TNFα, was assessed in the cortex, the hippocampus, the white matter, and the cerebellum using qPCR and Droplet Digital PCR. Exposure to hypoxia–reoxygenation significantly altered the transcription levels of BDNF-AS, H19, MALAT1, and ANRIL. BDNF-AS levels were significantly enhanced after both hypoxia and subsequent hyperoxic reoxygenation, 8% and 100% O2, respectively. Our observations suggest an emerging role for lncRNAs as part of the molecular response to hypoxia-induced damages during perinatal asphyxia. A better understanding of the regulatory properties of BDNF-AS and other lncRNAs may reveal novel targets and intervention strategies in the future.en_US
dc.language.isoengen_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleLong Non-Coding RNAs in Hypoxia and Oxidative Stress: Novel Insights Investigating a Piglet Model of Perinatal Asphyxiaen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.description.versionpublishedVersionen_US
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1
dc.identifier.doi10.3390/biology12040549
dc.identifier.cristin2141610
dc.source.journalBiology (Basel)en_US
dc.source.volume12en_US
dc.source.issue4en_US
dc.source.pagenumber15en_US


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Navngivelse 4.0 Internasjonal
Except where otherwise noted, this item's license is described as Navngivelse 4.0 Internasjonal