dc.contributor.author | Skogvold, Hanne Bendiksen | |
dc.contributor.author | Sandås, Elise Mørk | |
dc.contributor.author | Østeby, Anja | |
dc.contributor.author | Løkken, Camilla | |
dc.contributor.author | Rootwelt, Helge | |
dc.contributor.author | Rønning, Per Ola | |
dc.contributor.author | Wilson, Steven Ray Haakon | |
dc.contributor.author | Elgstøen, Katja Benedikte Prestø | |
dc.date.accessioned | 2021-10-12T07:29:10Z | |
dc.date.available | 2021-10-12T07:29:10Z | |
dc.date.created | 2021-08-23T12:56:07Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | Journal of Proteome Research. 2021, 20 (8), 4010-4021. | en_US |
dc.identifier.issn | 1535-3893 | |
dc.identifier.uri | https://hdl.handle.net/11250/2789130 | |
dc.description.abstract | Dried blood spot (DBS) metabolite analysis is a central tool for the clinic, e.g., newborn screening. Instead of applying multiple analytical methods, a single liquid chromatography-mass spectrometry (LC–MS) method was developed for metabolites spanning from highly polar glucose to hydrophobic long-chain acylcarnitines. For liquid chromatography, a diphenyl column and a multi-linear solvent gradient operated at elevated flow rates allowed for an even-spread resolution of diverse metabolites. Injecting moderate volumes of DBS organic extracts directly, in contrast to evaporation and reconstitution, provided substantial increases in analyte recovery. Q Exactive MS settings were also tailored for sensitivity increases, and the method allowed for analyte retention time and peak area repeatabilities of 0.1–0.4 and 2–10%, respectively, for a wide polarity range of metabolites (log P −4.4 to 8.8). The method’s performance was suited for both untargeted analysis and targeted approaches evaluated in clinically relevant experiments. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | American Chemical Society | en_US |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.subject | Metabolomics | en_US |
dc.subject | Dried blood spots | en_US |
dc.subject | Liquid chromatography-mass spectrometry | en_US |
dc.title | Bridging the polar and hydrophobicmMetabolome in single-run untargeted liquid chromatography-mass spectrometry dried blood spot metabolomics for clinical purposes | en_US |
dc.type | Peer reviewed | en_US |
dc.type | Journal article | en_US |
dc.description.version | publishedVersion | en_US |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 2 | |
dc.identifier.doi | 10.1021/acs.jproteome.1c00326 | |
dc.identifier.cristin | 1928022 | |
dc.source.journal | Journal of Proteome Research | en_US |
dc.source.volume | 20 | en_US |
dc.source.issue | 8 | en_US |
dc.source.pagenumber | 4010-4021 | en_US |