dc.contributor.author | Fladberg, Øyvind Andreas | |
dc.contributor.author | Jørgensen, Silje | |
dc.contributor.author | Aamot, Hege Vangstein | |
dc.date.accessioned | 2019-07-15T11:51:06Z | |
dc.date.available | 2019-07-15T11:51:06Z | |
dc.date.issued | 2017-11-29 | |
dc.identifier.citation | Fladberg, O. A., Jorgensen, S. B., & Aamot, H. V. (2017). Genotypic characterization of gentamicin and cephalosporin resistant Escherichia coli isolates from blood cultures in a Norwegian university hospital 2011-2015. Antimicrob Resist Infect Control, 6, 121. doi:10.1186/s13756-017-0280-2 | en |
dc.identifier.issn | 2047-2994 | |
dc.identifier.uri | https://hdl.handle.net/10642/7308 | |
dc.description.abstract | Background: Cephalosporin resistance in clinical E. coli isolates is increasing internationally. The increase has been caused by virulent and often multidrug-resistant clones, especially the extended spectrum beta-lactamase (ESBL) producing E. coli clone O25b-ST131. Methods: In Norway, recommended empirical treatment of sepsis consists of gentamicin and penicillin combined, or a broad-spectrum cephalosporin. To investigate if increased gentamicin and cephalosporins resistance rates in our hospital could be caused by specific clones, we conducted a retrospective study on E. coli blood culture isolates from 2011 through 2015. All E. coli isolates non-susceptible to gentamicin and/or third-generation cephalosporins were genotyped using multiple-locus variable-number of tandem repeat analysis (MLVA) and compared with antibiotic susceptible isolates. The frequency of the most common genes causing ESBL production (blaCTX-M, blaampC) was examined by Real-Time PCR. Results: A total of 158 cephalosporin and/or gentamicin resistant and 97 control isolates were differentiated into 126 unique MLVA types. Of these, 31% of the isolates belonged to a major MLVA cluster consisting of 41% of the gentamicin resistant and 35% of the cephalosporin resistant isolates. The majority (65/80 isolates) of this MLVA cluster contained MLVA types associated with the E. coli O25b-ST131 clone. Genes encoding CTX-M enzyme phylogroups 1 and 9 occurred in 65% and 19% of cephalosporin resistant isolates, respectively, whereas blaampC-CIT was identified in 3%. Conclusion: No local E. coli bacteraemia clone was identified. Antibiotic resistance was dispersed over a variety of genotypes. However, association with the international E. coli O25b-ST131 clone was frequent and may be an important driver behind increased resistance rates. Monitoring and preventing dissemination of these resistant clones are important for continued optimal treatment. | en |
dc.language.iso | en | en |
dc.publisher | BMC Springer | en |
dc.relation.ispartofseries | Antimicrobial Resistance & Infection Control;121(6) | |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/us/ | * |
dc.subject | VDP::Medisinske Fag: 700 | en |
dc.subject | Artikkel | en |
dc.title | Genotypic characterization of gentamicin and cephalosporin 1 resistant Escherichia coli 2 isolates from blood cultures in a Norwegian university hospital 2011-2015 | en |
dc.type | Journal article | en |
dc.type | Peer reviewed | en |
dc.description.version | acceptedVersion | en |
dc.identifier.doi | http://dx.doi.org/10.1186/s13756-017-0280-2 | |
dc.identifier.cristin | 1541811 | |