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dc.contributor.authorNygaard, Anders Benteson
dc.contributor.authorCharnock, Colin
dc.date.accessioned2017-09-27T12:19:44Z
dc.date.accessioned2017-10-04T10:38:47Z
dc.date.available2017-09-27T12:19:44Z
dc.date.available2017-10-04T10:38:47Z
dc.date.issued2017
dc.identifier.citationNygaard A, Charnock C. The bacterial composition of ventilation filter dust in Norwegian pre-school nurseries. Indoor + Built Environment. 2017language
dc.identifier.issn1420-326X
dc.identifier.issn1423-0070
dc.identifier.urihttps://hdl.handle.net/10642/5274
dc.description.abstractThe microbial content of dust collected from intake and exhaust air filters in six Norwegian nurseries was determined using 16S rRNA pyrosequencing and plate count analyses. The concentration of endotoxins was also estimated. About 96% of the sequences were classified as Actinobacteria, Proteobacteria, Firmicutes, Bacteroidetes and Cyanobacteria. At the genus level, about 30% of sequences from the exhaust filter were classified as bacteria of probable human origin, such as Streptococcus and Corynebacterium species. These were close to absent in intake dust samples (<1%). This suggests that occupancy shapes the indoor microbiota, creating an environment relatively rich in genera of potential health significance. There were significantly greater counts of culturable bacteria and fungi in exhaust samples, indicating that passage of air through the nursery causes deterioration in the general air quality. Although there was more endotoxin in exhaust dust, the endotoxin levels per colony forming unit were similar in both samples. This study explores, for the first time, the bacterial composition of ventilation filter dust in Norwegian nurseries, and is important as it reveals what types of microorganisms nursery users are exposed to. In addition to possible direct health issues, the nature of our early exposure to microbes may be significant in the development of immunological conditions.language
dc.language.isoenlanguage
dc.publisherSagelanguage
dc.subjectNorwaylanguage
dc.subjectVentilationlanguage
dc.subjectNurserieslanguage
dc.subjectAir qualitylanguage
dc.subjectIndoor ecologylanguage
dc.titleThe bacterial composition of ventilation filter dust in Norwegian pre-school nurserieslanguage
dc.typeJournal articlelanguage
dc.typePeer reviewedlanguage
dc.date.updated2017-09-27T12:19:44Z
dc.description.versionacceptedVersionlanguage
dc.identifier.doihttp://doi.org/10.1177/1420326X17713831
dc.identifier.cristin1475123
dc.source.journalIndoor + Built Environment
dc.subject.nsiVDP::Matematikk og naturvitenskap: 400::Basale biofag: 470::Generell mikrobiologi: 472
dc.subject.nsiVDP::Mathematics and natural scienses: 400::Basic biosciences: 470::General microbiology: 472


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