dc.contributor.author | Kruger, Kristi | |
dc.contributor.author | Wik, Elisabeth | |
dc.contributor.author | Knutsvik, Gøril | |
dc.contributor.author | Nalwoga, Hawa | |
dc.contributor.author | Klingen, Tor Audun | |
dc.contributor.author | Arnes, Jarle | |
dc.contributor.author | Chen, Ying | |
dc.contributor.author | Mannelqvist, Monica | |
dc.contributor.author | Dimitrakopoulou, Konstantina | |
dc.contributor.author | Stefansson, Ingunn | |
dc.contributor.author | Birkeland, Even | |
dc.contributor.author | Aas, Turid | |
dc.contributor.author | Tobin, Nicholas P | |
dc.contributor.author | Jonassen, Inge | |
dc.contributor.author | Bergh, Jonas | |
dc.contributor.author | Foulkes, William D | |
dc.contributor.author | Akslen, Lars A | |
dc.date.accessioned | 2019-07-12T10:53:05Z | |
dc.date.available | 2019-07-12T10:53:05Z | |
dc.date.issued | 2017-04-24 | |
dc.identifier.citation | Krüger, K., Wik, E., Knutsvik, G., Nalwoga, H., Klingen, T. A., Arnes, J. B., ... & Birkeland, E. (2017). Expression of Nestin associates with BRCA1 mutations, a basal-like phenotype and aggressive breast cancer. Scientific reports, 7(1), 1089. | en |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | https://hdl.handle.net/10642/7287 | |
dc.description.abstract | We here examined whether Nestin, by protein and mRNA levels, could be a predictor of BRCA1 related breast cancer, a basal-like phenotype, and aggressive tumours. Immunohistochemical staining of Nestin was done in independent breast cancer hospital cohorts (Series I-V, total 1257 cases). Also, TCGA proteomic data (n = 103), mRNA microarray data from TCGA (n = 520), METABRIC (n = 1992), and 6 open access breast cancer datasets (n = 1908) were analysed. Patients with Nestin protein expression in tumour cells more often had BRCA1 germline mutations (OR 8.7, p < 0.0005, Series III), especially among younger patients (<40 years at diagnosis) (OR 16.5, p = 0.003). Nestin protein positivity, observed in 9–28% of our hospital cases (Series I-IV), was independently associated with reduced breast cancer specific survival (HR = 2.0, p = 0.035) and was consistently related to basal-like differentiation (by Cytokeratin 5, OR 8.7–13.8, p < 0.0005; P-cadherin OR 7.0–8.9, p < 0.0005; EGFR staining, OR 3.7–8.2, p ≤ 0.05). Nestin mRNA correlated significantly with Nestin protein expression (ρ = 0.6, p < 0.0005), and high levels were seen in the basal-like intrinsic subtype. Gene expression signalling pathways linked to high Nestin were explored, and revealed associations with stem-like tumour features. In summary, Nestin was strongly associated with germline BRCA1 related breast cancer, a basal-like phenotype, reduced survival, and stemness characteristics. | en |
dc.language.iso | en | en |
dc.publisher | Nature | en |
dc.relation.ispartofseries | Scientific Reports;7(1) | |
dc.rights | Attribution 3.0 United States
This is an open acces article, originally published at https://doi.org/10.1038/s41598-017-00862-w | en |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/us/ | * |
dc.subject | VDP::Medisinske Fag: 700 | en |
dc.subject | Artikkel | en |
dc.title | Expression of Nestin associates with BRCA1 mutations, a basal-like phenotype and aggressive breast cancer | en |
dc.type | Journal article | en |
dc.type | Peer reviewed | en |
dc.description.version | publishedVersion | en |
dc.identifier.doi | https://doi.org/10.1038/s41598-017-00862-w | |
dc.identifier.cristin | 1466633 | |