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dc.contributor.authorØstbye, Tone-Kari K
dc.contributor.authorWoldemariam, Nardos Tesfaye
dc.contributor.authorLundberg, Camilla S.
dc.contributor.authorBerge, Gerd Marit
dc.contributor.authorRuyter, Bente
dc.contributor.authorAndreassen, Rune
dc.date.accessioned2021-01-24T20:23:52Z
dc.date.accessioned2021-03-02T09:40:02Z
dc.date.available2021-01-24T20:23:52Z
dc.date.available2021-03-02T09:40:02Z
dc.date.issued2020-12-17
dc.identifier.citationØstbye Tk, Woldemariam NT, Lundberg CS, Berge Gm, Ruyter B, Andreassen R. Modulation of hepatic miRNA expression in Atlantic salmon (Salmo salar) by family background and dietary fatty acid composition. Journal of Fish Biology. 2020en
dc.identifier.issn0022-1112
dc.identifier.issn1095-8649
dc.identifier.urihttps://hdl.handle.net/10642/9798
dc.description.abstractThis study finds significant differences in hepatic fatty acid composition between four groups of Atlantic salmon (Salmo salar) consisting of offspring from families selected for high and low capacities to express the delta 6 desaturase isomer b and fed diets with 10% or 75% fish oil. The results demonstrated that hepatic lipid metabolism was affected by the experimental conditions (diet/family). The fatty acid composition in the four groups mirrored the dietary composition differences, but it was also associated with the family groups. Small RNA sequencing followed by RT-qPCR identified twelve differentially expressed miRNAs (DE miRNAs) with expression associated with family groups (miR-146 family members, miR-200b, miR-214, miR-221, miR-125, miR-135, miR-137, miR_nov_1), diets (miR-203, miR-462) or both conditions. All the conserved DE miRNAs have been reported as associated with lipid metabolism in other vertebrates. In silico predictions revealed 37 lipid metabolism pathway genes including desaturases, transcription factors and key enzymes in the synthesis pathways as putative targets (e.g.srebp-1 and 2, Δ6fad_b and c, hmdh, elovl4 and 5b, cdc42). RT-qPCR analysis of selected target genes showed expression changes that were associated with diet and with family groups (d5fad, d6fad_a, srebp-1). There was reciprocal difference in abundance of ssa-miR-203a-3p and srebp-1 in one group comparison, while other predicted targets did not reveal any evidence of being negatively regulated by degradation. More experimental studies are needed to validate and fully understand the predicted interactions and how the DE miRNAs may participate in regulation of hepatic lipid metabolism.en
dc.description.sponsorshipNorges Forskningsråd, Grant/Award Numbers: 280839/E40, 254849/E90en
dc.language.isoenen
dc.publisherWileyen
dc.relation.ispartofseriesJournal of Fish Biology;
dc.rightsCreative Commons Attribution 4.0 International (CC BY 4.0) Licenseen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectAtlantic salmonen
dc.subjectDietsen
dc.subjectLipid metabolismen
dc.subjectmiRNAen
dc.subjectSmall-RNA sequencingen
dc.titleModulation of hepatic miRNA expression in Atlantic salmon (Salmo salar) by family background and dietary fatty acid compositionen
dc.typeJournal articleen
dc.typePeer revieweden
dc.date.updated2021-01-24T20:23:52Z
dc.description.versionpublishedVersionen
dc.identifier.doihttps://doi.org/10.1111/jfb.14649
dc.identifier.cristin1861679
dc.source.journalJournal of Fish Biology
dc.relation.projectIDNorges forskningsråd: 254849
dc.relation.projectIDNorges forskningsråd: 280839
dc.relation.projectIDDirektoratet for internasjonalisering og kvalitetsutvikling i høgare utdanning: HPN-2015-10026


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