dc.contributor.author | Aas, Vigdis | |
dc.contributor.author | Thoresen, G. Hege | |
dc.contributor.author | Rustan, Arild | |
dc.contributor.author | Lund, Jenny | |
dc.date.accessioned | 2020-11-15T15:24:29Z | |
dc.date.accessioned | 2021-02-10T16:52:23Z | |
dc.date.available | 2020-11-15T15:24:29Z | |
dc.date.available | 2021-02-10T16:52:23Z | |
dc.date.issued | 2020-09-08 | |
dc.identifier.citation | Aas V, Thoresen GH, Rustan A, Lund J. Substrate oxidation in primary human skeletal muscle cells is influenced by donor age. Cell and Tissue Research. 2020 | en |
dc.identifier.issn | 0302-766X | |
dc.identifier.issn | 1432-0878 | |
dc.identifier.uri | https://hdl.handle.net/10642/9510 | |
dc.description.abstract | Primary human myotubes represent an alternative system to intact skeletal muscle for the study of human diseases related to
changes in muscle energy metabolism. This work aimed to study if fatty acid and glucose metabolism in human myotubes in vitro
were related to muscle of origin, donor gender, age, or body mass index (BMI). Myotubes from a total of 82 donors were
established from three different skeletal muscles, i.e., musculus vastus lateralis, musculus obliquus internus abdominis, and
musculi interspinales, and cellular energy metabolism was evaluated. Multiple linear regression analyses showed that donor age
had a significant effect on glucose and oleic acid oxidation after correcting for gender, BMI, and muscle of origin. Donor BMI
was the only significant contributor to cellular oleic acid uptake, whereas cellular glucose uptake did not rely on any of the
variables examined. Despite the effect of age on substrate oxidation, cellular mRNA expression of pyruvate dehydrogenase
kinase 4 (PDK4) and peroxisome proliferator–activated receptor gamma coactivator 1 alpha (PPARGC1A) did not correlate with
donor age. In conclusion, donor age significantly impacts substrate oxidation in cultured human myotubes, whereas donor BMI
affects cellular oleic acid uptake. | en |
dc.description.sponsorship | Open Access funding provided by University of Oslo (incl Oslo University Hospital). This work was funded by research grants from OsloMet - Oslo Metropolitan University, the University of Oslo, the Norwegian Diabetes Association, and Anders Jahre’s Foundation. | en |
dc.language.iso | en | en |
dc.publisher | Springer | en |
dc.relation.ispartofseries | Cell and Tissue Research;382 (2020) | |
dc.rights | Creative Commons Attribution 4.0 International (CC BY 4.0) License | en |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.subject | Skeletal muscle cells | en |
dc.subject | Energy metabolisms | en |
dc.subject | Ages | en |
dc.subject | Body mass index | en |
dc.subject | Origin muscles | en |
dc.subject | Genders | en |
dc.title | Substrate oxidation in primary human skeletal muscle cells is influenced by donor age | en |
dc.type | Journal article | |
dc.type | Peer reviewed | en |
dc.date.updated | 2020-11-15T15:24:29Z | |
dc.description.version | publishedVersion | en |
dc.identifier.doi | https://doi.org/10.1007/s00441-020-03275-w | |
dc.identifier.cristin | 1828462 | |
dc.source.journal | Cell and Tissue Research | |