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dc.contributor.authorShwe, Alice
dc.contributor.authorØstbye, Tone-Kari K
dc.contributor.authorKrasnov, Aleksei
dc.contributor.authorRamberg, Sigmund
dc.contributor.authorAndreassen, Rune
dc.date.accessioned2020-09-15T14:41:48Z
dc.date.accessioned2021-01-21T13:52:42Z
dc.date.available2020-09-15T14:41:48Z
dc.date.available2021-01-21T13:52:42Z
dc.date.issued2020-09-08
dc.identifier.citationShwe A, Østbye Tk, Krasnov A, Ramberg S, Andreassen R. Characterization of Differentially Expressed miRNAs and Their Predicted Target Transcripts during Smoltification and Adaptation to Seawater in Head Kidney of Atlantic Salmon. Genes. 2020;11:1059en
dc.identifier.issn2073-4425
dc.identifier.issn2073-4425
dc.identifier.urihttps://hdl.handle.net/10642/9381
dc.description.abstractSmoltification and early seawater phase are critical developmental periods with physiological and biochemical changes in Atlantic salmon that facilitates survival in saltwater. MicroRNAs (miRNAs) are known to have important roles in development, but whether any miRNAs are involved in regulation of gene expression during smoltification and the adaption to seawater is largely unknown. Here, small RNA sequencing of materials from head kidney before, during smoltification and post seawater transfer were used to study expression dynamics of miRNAs, while microarray analysis was applied to study mRNA expression dynamics. Comparing all timepoints, 71 miRNAs and 2709 mRNAs were identified as differentially expressed (DE). Hierarchical clustering analysis of the DE miRNAs showed three major clusters with characteristic expression changes. Eighty-one DE mRNAs revealed negatively correlated expression patterns to DE miRNAs in Cluster I and III. Furthermore, 42 of these mRNAs were predicted as DE miRNA targets. Gene enrichment analysis of negatively correlated target genes showed they were enriched in gene ontology groups hormone biosynthesis, stress management, immune response, and ion transport. The results strongly indicate that post-transcriptional regulation of gene expression by miRNAs is important in smoltification and sea water adaption, and this study identifies several putative miRNA-target pairs for further functional studies.en
dc.description.sponsorshipThis research was funded by a Norwegian Research Council grant (280839/E40) to R.A.en
dc.language.isoenen
dc.publisherMDPIen
dc.relation.ispartofseriesGenes;Volume 11, Issue 9
dc.rightsCreative Commons Attribution 4.0 International (CC BY 4.0) licenseen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectSmoltificationen
dc.subjectParr-smolt transformationsen
dc.subjectMicroRNAsen
dc.subjectSeawater adaptationsen
dc.subjectAtlantic salmonen
dc.subjectHead kidneysen
dc.subjectMicroarraysen
dc.titleCharacterization of Differentially Expressed miRNAs and Their Predicted Target Transcripts during Smoltification and Adaptation to Seawater in Head Kidney of Atlantic Salmonen
dc.typeJournal articleen
dc.typePeer revieweden
dc.date.updated2020-09-15T14:41:48Z
dc.description.versionpublishedVersionen
dc.identifier.doihttps://doi.org/10.3390/genes11091059
dc.identifier.cristin1830188
dc.source.journalGenes
dc.relation.projectIDNorges forskningsråd: 280839


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