Increased protein intake affects pro-opiomelanocortin (POMC) processing, immune function and IGF signaling in peripheral blood mononuclear cells of home-dwelling old subjects using a genome-wide gene expression approach
dc.contributor.author | Ulven, Stine Marie | |
dc.contributor.author | Holven, Kirsten Bjørklund | |
dc.contributor.author | Rundblad, Amanda | |
dc.contributor.author | Flatberg, Arnar | |
dc.contributor.author | Myhrstad, Mari | |
dc.contributor.author | Karlsen, Karina | |
dc.contributor.author | Mutt, Shivaprakash | |
dc.contributor.author | Herzig, Karl-Heinz | |
dc.contributor.author | Ottestad, Inger | |
dc.contributor.author | Gjevestad, Gyrd Omholt | |
dc.date.accessioned | 2020-01-28T08:18:31Z | |
dc.date.accessioned | 2020-04-13T22:03:53Z | |
dc.date.available | 2020-01-28T08:18:31Z | |
dc.date.available | 2020-04-13T22:03:53Z | |
dc.date.issued | 2019-11-28 | |
dc.identifier.citation | Ulven S, Holven KB, Rundblad A, Flatberg A, Myhrstad MC, Karlsen, Mutt, Herzig K, Ottestad I, Gjevestad GOG. Increased protein intake affects proopiomelanocortin (POMC) processing, immune function and IGF signaling in peripheral blood mononuclear cells of home-dwelling old subjects using a genome-wide gene expression approach. Genes & Nutrition. 2019;14(32) | en |
dc.identifier.issn | 1555-8932 | |
dc.identifier.issn | 1555-8932 | |
dc.identifier.issn | 1865-3499 | |
dc.identifier.uri | https://hdl.handle.net/10642/8418 | |
dc.description.abstract | Background: Adequate protein intake among older adults is associated with better health outcomes such as immune function and metabolic regulation of skeletal muscle, but conflicting results make it difficult to define the optimal intake. To further understand the impact of protein intake on metabolic processes, the aim of the study was to explore genome-wide gene expression changes in peripheral blood mononuclear cells (PBMCs) in homedwelling old subjects after increased protein intake for 12weeks. Method: In a parallel double-blind randomized controlled intervention study, subjects (≥ 70years) received a protein-enriched milk (2 × 20g protein/day, n = 14, mean (±SD) age 76.9 ± 4.9years) or an isocaloric carbohydrate drink (n = 17, mean (±SD) age 77.7 ± 4.8years) for breakfast and evening meal for 12weeks. PBMCs were isolated before and after the intervention. Microarray analysis was performed using Illumina technology. Serum levels of gut peptides and insulin growth factor (IGF)-1 were also measured. Results: In total 758 gene transcripts were regulated after increased protein intake, and 649 gene transcripts were regulated after intake of carbohydrates (p < 0.05). Forty-two of these genes were overlapping. After adjusting for multiple testing, 27 of the 758 gene transcripts were regulated (FDR, q-value < 0.25) after protein intake. Of these 25 were upregulated and two downregulated. In particular, genes and signaling pathways involved in proopiomelanocortin (POMC) processing, immune function, and IGF signaling were significantly altered. Conclusions: PBMCs can be used to study gene expression changes after long-term protein intake, as many signaling pathways were regulated after increased protein intake. The functional significance of these findings needs to be further investigated. Trial registration: ClinicalTrials.gov, ID no. NCT02218333. The study was registered on August 18, 2014. | en |
dc.description.sponsorship | The Research Council of Norway (225258/E.40), Throne Holst Foundation for Nutrition Research, University of Oslo, and TINE SA supported this work. | en |
dc.language.iso | en | en |
dc.publisher | BMC | en |
dc.relation.ispartofseries | Genes and Nutrition;14, Article number: 32 (2019) | |
dc.rights | This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. | en |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.subject | Older adults | en |
dc.subject | Randomized controlled trials | en |
dc.subject | Dairy proteins | en |
dc.subject | Peripheral mononuclear cells | en |
dc.subject | Transcriptomics | en |
dc.subject | Immune functions | en |
dc.title | Increased protein intake affects pro-opiomelanocortin (POMC) processing, immune function and IGF signaling in peripheral blood mononuclear cells of home-dwelling old subjects using a genome-wide gene expression approach | en |
dc.type | Journal article | en |
dc.type | Peer reviewed | en |
dc.date.updated | 2020-01-28T08:18:31Z | |
dc.description.version | publishedVersion | en |
dc.identifier.doi | https://dx.doi.org/10.1186/s12263-019-0654-6 | |
dc.identifier.cristin | 1783684 | |
dc.source.journal | Genes & Nutrition |
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Except where otherwise noted, this item's license is described as This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.