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dc.contributor.authorLund, Jenny
dc.contributor.authorOuwens, DM
dc.contributor.authorWettergreen, Marianne
dc.contributor.authorBakke, Siril Skaret
dc.contributor.authorThoresen, G. Hege
dc.contributor.authorAas, Vigdis
dc.date.accessioned2020-01-14T08:33:43Z
dc.date.accessioned2020-03-17T12:03:35Z
dc.date.available2020-01-14T08:33:43Z
dc.date.available2020-03-17T12:03:35Z
dc.date.issued2019-09-18
dc.identifier.citationLund J, Ouwens, Wettergreen M, Bakke SS, Thoresen GH, Aas V. Increased glycolysis and higher lactate production in hyperglycemic myotubes. Cells. 2019;8(9)en
dc.identifier.issn2073-4409
dc.identifier.issn2073-4409
dc.identifier.urihttps://hdl.handle.net/10642/8287
dc.description.abstractPrevious studies have shown that chronic hyperglycemia impairs glucose and fatty acid oxidation in cultured human myotubes. To further study the hyperglycemia-induced suppression of oxidation, lactate oxidation, mitochondrial function and glycolytic rate were evaluated. Further, we examined the intracellular content of reactive oxygen species (ROS), production of lactate and conducted pathway-ANOVA analysis on microarray data. In addition, the roles of the pentose phosphate pathway (PPP) and the hexosamine pathway were evaluated. Lactic acid oxidation was suppressed in hyperglycemic versus normoglycaemic myotubes. No changes in mitochondrial function or ROS concentration were observed. Pathway-ANOVA analysis indicated several upregulated pathways in hyperglycemic cells, including glycolysis and PPP. Functional studies showed that glycolysis and lactate Production were higher in hyperglycemic than normoglycaemic cells. However, there were no indications of involvement of PPP or the hexosamine pathway. In conclusion, hyperglycemia reduced substrate oxidation while increasing glycolysis and lactate production in cultured human myotubes.en
dc.language.isoenen
dc.publisherMDPIen
dc.relation.ispartofseriesCells;Volume 8, Issue 9
dc.rights© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectHyperglycemiaen
dc.subjectMyotubesen
dc.subjectGlycolysesen
dc.subjectLactatesen
dc.subjectSubstrate oxidationsen
dc.subjectPathway analysesen
dc.titleIncreased glycolysis and higher lactate production in hyperglycemic myotubesen
dc.typeJournal articleen
dc.typePeer revieweden
dc.date.updated2020-01-14T08:33:43Z
dc.description.versionpublishedVersionen
dc.identifier.doihttp://dx.doi.org/10.3390/cells8091101
dc.identifier.cristin1772006
dc.source.journalCells


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© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Med mindre annet er angitt, så er denne innførselen lisensiert som © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).