Quantitative proteomic analyses of CD4+ and CD8+ T cells reveal differentially expressed proteins in multiple sclerosis patients and healthy controls
dc.contributor.author | Berge, Tone | |
dc.contributor.author | Eriksson, Anna | |
dc.contributor.author | Brorson, Ina Skaara | |
dc.contributor.author | Høgestøl, Einar August | |
dc.contributor.author | Berg-Hansen, Pål | |
dc.contributor.author | Døskeland, Anne Marie Simonne | |
dc.contributor.author | Mjaavatten, Olav | |
dc.contributor.author | Bos, Steffan Daniel | |
dc.contributor.author | Harbo, Hanne Flinstad | |
dc.contributor.author | Berven, Frode | |
dc.date.accessioned | 2019-11-13T12:59:32Z | |
dc.date.accessioned | 2019-11-14T13:52:37Z | |
dc.date.available | 2019-11-13T12:59:32Z | |
dc.date.available | 2019-11-14T13:52:37Z | |
dc.date.issued | 2019-04-27 | |
dc.identifier.citation | Berge T, Eriksson A, Brorson IS, Høgestøl EA, Berg-Hansen PBH, Døskeland AP, Mjaavatten O, Bos SD, Harbo HFH, Berven F. Quantitative proteomic analyses of CD4+ and CD8+ T cells reveal differentially expressed proteins in multiple sclerosis patients and healthy controls . Clinical Proteomics. 2019;16:19:1-18 | en |
dc.identifier.issn | 1542-6416 | |
dc.identifier.issn | 1559-0275 | |
dc.identifier.issn | 1559-0275 | |
dc.identifier.uri | https://hdl.handle.net/10642/7829 | |
dc.description.abstract | Background: Multiple sclerosis (MS) is an autoimmune, neuroinflammatory disease, with an unclear etiology. How‑ ever, T cells play a central role in the pathogenesis by crossing the blood–brain‑barrier, leading to inflammation of the central nervous system and demyelination of the protective sheath surrounding the nerve fibers. MS has a complex inheritance pattern, and several studies indicate that gene interactions with environmental factors contribute to disease onset. Methods: In the current study, we evaluated T cell dysregulation at the protein level using electrospray liquid chro‑ matography–tandem mass spectrometry to get novel insights into immune‑cell processes in MS. We have analyzed the proteomic profiles of CD4+ and CD8+ T cells purified from whole blood from 13 newly diagnosed, treatment‑ naive female patients with relapsing–remitting MS and 14 age‑ and sex‑matched healthy controls. Results: An overall higher protein abundance was observed in both CD4+ and CD8+ T cells from MS patients when compared to healthy controls. The differentially expressed proteins were enriched for T‑cell specific activation path‑ ways, especially CTLA4 and CD28 signaling in CD4+ T cells. When selectively analyzing proteins expressed from the genes most proximal to > 200 non‑HLA MS susceptibility polymorphisms, we observed differential expression of eight proteins in T cells between MS patients and healthy controls, and there was a correlation between the genotype at three MS genetic risk loci and protein expressed from proximal genes. Conclusion: Our study provides evidence for proteomic differences in T cells from relapsing–remitting MS patients compared to healthy controls and also identifies dysregulation of proteins encoded from MS susceptibility genes. | en |
dc.description.sponsorship | The study was funded by the South Eastern Norway Regional Health Authority (Grant No. 2017114), the Norwegian Research Council (Grant No. 240102), OsloMet – Oslo Metropolitan University, Biogen, Sanofi Genzyme and the Odd Fellow Society. | en |
dc.language.iso | en | en |
dc.publisher | BMC (part of Springer Nature) | en |
dc.relation.ispartofseries | Clinical Proteomics;16, Article number: 19 (2019) | |
dc.rights | This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creat iveco mmons .org/licen ses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. | en |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.subject | Multiple sclerosis | en |
dc.subject | T cells | en |
dc.subject | Mass spectrometry | en |
dc.subject | Single nucleotide polymorphisms | en |
dc.subject | Autoimmunity | en |
dc.subject | Proteomics | en |
dc.title | Quantitative proteomic analyses of CD4+ and CD8+ T cells reveal differentially expressed proteins in multiple sclerosis patients and healthy controls | en |
dc.type | Peer reviewed | en |
dc.type | Journal article | |
dc.date.updated | 2019-11-13T12:59:32Z | |
dc.description.version | publishedVersion | en |
dc.identifier.doi | https://dx.doi.org/10.1186/s12014-019-9241-5 | |
dc.identifier.cristin | 1709560 | |
dc.source.journal | Clinical Proteomics | |
dc.relation.projectID | Norges forskningsråd: 240102 | |
dc.relation.projectID | Helse Sør-Øst RHF: 2017114 |
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Med mindre annet er angitt, så er denne innførselen lisensiert som This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creat iveco mmons .org/licen ses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.