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dc.contributor.authorLarsen, Sunniva Veen
dc.contributor.authorHolven, Kirsten Bjørklund
dc.contributor.authorOttestad, Inger
dc.contributor.authorDagsland, Kine Margrethe
dc.contributor.authorMyhrstad, Mari
dc.contributor.authorUlven, Stine Marie
dc.date.accessioned2019-01-31T10:50:04Z
dc.date.accessioned2019-03-14T14:58:56Z
dc.date.available2019-01-31T10:50:04Z
dc.date.available2019-03-14T14:58:56Z
dc.date.issued2018-04-10
dc.identifier.citationLarsen SV, Holven KB, Ottestad I, Dagsland, Myhrstad MC, Ulven S. Plasma fatty acid levels and gene expression related to lipid metabolism in peripheral blood mononuclear cells: A cross-sectional study in healthy subjects. Genes & Nutrition. 2018;13(1)en
dc.identifier.issn1555-8932
dc.identifier.issn1555-8932
dc.identifier.issn1865-3499
dc.identifier.urihttps://hdl.handle.net/10642/6775
dc.description.abstractBackground: Solid evidence indicates that intake of marine n-3 fatty acids lower serum triglycerides, and that replacing saturated fatty acids (SFA) with polyunsaturated fatty acids (PUFA) reduces plasma total cholesterol and LDL-cholesterol. The molecular mechanisms underlying these health beneficial effects are however not completely elucidated. The aim of this study was therefore to investigate the expression of genes related to lipid metabolism in peripheral blood mononuclear cells (PBMC) depending on the plasma levels of n-6 and n-3 39 fatty acids and the SFA to PUFA ratio. Methods: Fifty-four healthy subjects were grouped into tertiles (n=18) based on plasma levels of n-6 and n-3 fatty acids and the SFA to PUFA ratio. The PBMC gene expression levels among subjects in the highest versus the lowest tertiles were compared. In total, 285 genes related to cholesterol and triglyceride metabolism were selected for this explorative study. Results: Among the 285 selected genes, 161 were defined as expressed in the PBMCs. The 47 plasma SFA to PUFA ratio was associated with the highest number of significantly different expressed genes (25 gene transcripts), followed by plasma n-6 fatty acid level (15 gene transcripts) and plasma n-3 fatty acid level (8 gene transcripts). In particular, genes involved in cholesterol homeostasis were significantly different expressed among subjects with high compared to low plasma SFA to PUFA ratio. Conclusion: Genes involved in lipid metabolism were differentially expressed in PBMCs depending on the plasma fatty acid levels. This finding may increase our understanding of how fatty acids influence lipid metabolism at a molecular level in humans.en
dc.description.sponsorshipThis study was funded by the University of Oslo, Oslo and Akershus University College of Applied Science, The research council of Norway and the Throne-Holst Foundation for Nutrition Research, Oslo, Norway.en
dc.language.isoenen
dc.publisherBMCen
dc.relation.ispartofseriesGenes & Nutrition;2018 13:9
dc.subjectCardiovascular risk factorsen
dc.subjectNutritionen
dc.subjectPlasma fatty acidsen
dc.subjectLipid metabolismsen
dc.subjectGene expressionsen
dc.subjectPeripheral blood mononuclear cellsen
dc.titlePlasma fatty acid levels and gene expression related to lipid metabolism in peripheral blood mononuclear cells: A cross-sectional study in healthy subjectsen
dc.typeJournal articleen
dc.typePeer revieweden
dc.date.updated2019-01-31T10:50:04Z
dc.description.versionacceptedVersionen
dc.identifier.doihttp://dx.doi.org/10.1186/s12263-018-0600-z
dc.identifier.cristin1593901
dc.source.journalGenes & Nutrition


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