HPV Genotyping of Modified General Primer-Amplicons Is More Analytically Sensitive and Specific by Sequencing than by Hybridization
dc.contributor.author | Meisal, Roger | |
dc.contributor.author | Rounge, Trine Ballestad | |
dc.contributor.author | Christiansen, Irene Kraus | |
dc.contributor.author | Eieland, Alexander Kirkeby | |
dc.contributor.author | Worren, Merete Molton | |
dc.contributor.author | Molden, Tor | |
dc.contributor.author | Kommedal, Øyvind | |
dc.contributor.author | Hovig, Eivind | |
dc.contributor.author | Leegaard, Truls Michael | |
dc.contributor.author | Ambur, Ole Herman | |
dc.date.accessioned | 2017-11-21T12:10:26Z | |
dc.date.accessioned | 2018-01-23T13:32:24Z | |
dc.date.available | 2017-11-21T12:10:26Z | |
dc.date.available | 2018-01-23T13:32:24Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | Meisal R, Rounge TB, Christiansen IK, Eieland AK, Worren M, Molden T, Kommedal Ø, Hovig E, Leegaard TM, Ambur OH. HPV Genotyping of Modified General Primer-Amplicons Is More Analytically Sensitive and Specific by Sequencing than by Hybridization. PLoS ONE. 2017;12(1):1-14 | en |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | https://hdl.handle.net/10642/5559 | |
dc.description.abstract | Sensitive and specific genotyping of human papillomaviruses (HPVs) is important for population-based surveillance of carcinogenic HPV types and for monitoring vaccine effectiveness. Here we compare HPV genotyping by Next Generation Sequencing (NGS) to an established DNA hybridization method. In DNA isolated from urine, the overall analytical sensitivity of NGS was found to be 22% higher than that of hybridization. NGS was also found to be the most specific method and expanded the detection repertoire beyond the 37 types of the DNA hybridization assay. Furthermore, NGS provided an increased resolution by identifying genetic variants of individual HPV types. The same Modified General Primers (MGP)-amplicon was used in both methods. The NGS method is described in detail to facilitate implementation in the clinical microbiology laboratory and includes suggestions for new standards for detection and calling of types and variants with improved resolution. | en |
dc.language.iso | en | en |
dc.publisher | Public Library of Science | en |
dc.rights | Copyright: © 2017 Meisal et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | en |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | Human papillomavirus | en |
dc.subject | HPV-16 | en |
dc.subject | Polymerase chain reaction | en |
dc.subject | Urine | en |
dc.subject | Genotyping | en |
dc.subject | Next-generation sequencing | en |
dc.subject | Vaccines | en |
dc.subject | Probe hybridization | en |
dc.title | HPV Genotyping of Modified General Primer-Amplicons Is More Analytically Sensitive and Specific by Sequencing than by Hybridization | en |
dc.type | Journal article | en |
dc.type | Peer reviewed | en |
dc.date.updated | 2017-11-21T12:10:26Z | |
dc.description.version | publishedVersion | en |
dc.identifier.doi | http://doi.org/10.1371/journal.pone.0169074 | |
dc.identifier.cristin | 1441001 | |
dc.source.journal | PLoS ONE |
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HV - Institutt for naturvitenskapelige helsefag [392]
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Med mindre annet er angitt, så er denne innførselen lisensiert som Copyright: © 2017 Meisal et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.