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dc.contributor.authorRein, Idun Dale
dc.contributor.authorStokke, Caroline
dc.contributor.authorJalal, Marwa
dc.contributor.authorMyklebust, June H.
dc.contributor.authorPatzke, Sebastian
dc.contributor.authorStokke, Trond
dc.date.accessioned2016-04-20T11:17:23Z
dc.date.available2016-04-20T11:17:23Z
dc.date.issued2015-08-28
dc.identifier.citationDale Rein, I., Stokke, C., Jalal, M., Myklebust, J. H., Patzke, S., & Stokke, T. (2015). New distinct compartments in the G2 phase of the cell cycle defined by the levels of γH2AX. Cell Cycle, 14(20), 3261-3269.en_US
dc.identifier.issn1538-4101
dc.identifier.otherFRIDAID 1335348
dc.identifier.urihttp://dx.doi.org/10.1080/15384101.2015.1087617
dc.identifier.urihttps://hdl.handle.net/10642/3252
dc.description.abstractInduction of DNA double strand breaks leads to phosphorylation and focus-formation of H2AX. However, foci of phosphorylated H2AX (γH2AX) appear during DNA replication also in the absence of exogenously applied injury. We measured the amount and the number of foci of γH2AX in different phases of the cell cycle by flow cytometry, sorting and microscopy in 4 malignant B-lymphocyte cell lines. There were no detectable γH2AX and no γH2AX-foci in G1 cells in exponentially growing cells and cells treated with PARP inhibitor (PARPi) for 24 h to create damage and reduce DNA repair. The amount of γH2AX increased immediately upon S phase entry, and about 10 and 30 γH2AX foci were found in mid-S phase control and PARPi-treated cells, respectively. The γH2AX-labeled damage caused by DNA replication was not fully repaired before entry into G2. Intriguingly, G2 cells populated a continuous distribution of γH2AX levels, from cells with a high content of γH2AX and the same number of foci as S phase cells (termed “G2H” compartment), to cells that there were almost negative and had about 2 foci (termed “G2L” compartment). EdU-labeling of S phase cells revealed that G2H was directly populated from S phase, while G2L was populated from G2H, but in control cells also directly from S phase. The length of G2H in particular increased after PARPi treatment, compatible with longer DNA-repair times. Our results show that cells repair replication-induced damage in G2H, and enter mitosis after a 2–3 h delay in G2L.en_US
dc.language.isoengen_US
dc.publisherTaylor & Francisen_US
dc.relation.ispartofseriesCell Cycle;14(20)
dc.subjectDNA repairen_US
dc.subjectG2 cell cycle phaseen_US
dc.subjectionizing radiationen_US
dc.subjectPARP inhibitionen_US
dc.subjectyH2AXen_US
dc.subjectVDP::Teknologi: 500::Medisinsk teknologi: 620en_US
dc.titleNew distinct compartments in the G2 phase of the cell cycle defined by the levels of γH2AX.en_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US
dc.description.versionThis is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/ by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.en_US


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