Myotubes from Severely Obese Type 2 Diabetic Subjects Accumulate Less Lipids and Show Higher Lipolytic Rate than Myotubes from Severely Obese Non-Diabetic Subjects
Journal article, Peer reviewed
Copyright: © 2015 bakke et al. this is an open access article distributed under the terms of the creative commons attribution license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
MetadataShow full item record
Original versionBakke, S. S., Feng, Y. Z., Nikolić, N., Kase, E. T., Moro, C., Stensrud, C., ... & Rustan, A. C. (2015). Myotubes from severely obese type 2 diabetic subjects accumulate less lipids and show higher lipolytic rate than myotubes from severely obese non-diabetic subjects. PloS one, 10(3), e0119556. http://dx.doi.org/10.1371/journal.pone.0119556
About 80% of patients with type 2 diabetes are classified as overweight. However, onlyabout 1/3 of severely obese subjects have type 2 diabetes. This indicates that several se-verely obese individuals may possess certain characteristics that protect them against type2 diabetes. We therefore hypothesized that this apparent paradox could be related to funda-mental differences in skeletal muscle lipid handling. Energy metabolism and metabolic flexi-bility were examined in human myotubes derived from severely obese subjects without(BMI 44±7 kg/m2) and with type 2 diabetes (BMI 43±6 kg/m2). Lower insulin sensitivity wasobserved in myotubes from severely obese subjects with type 2 diabetes. Lipolysis ratewas higher, and oleic acid accumulation, triacylglycerol content, and fatty acid adaptabilitywere lower in myotubes from severely obese subjects with type 2 diabetes compared to se-verely obese non-diabetic subjects. There were no differences in lipid distribution andmRNA and protein expression of the lipases HSL and ATGL, the lipase cofactor CGI-58, orthe lipid droplet proteins PLIN2 and PLIN3. Glucose and oleic acid oxidation were also simi-lar in cells from the two groups. In conclusion, myotubes established from severely obesedonors with established type 2 diabetes had lower ability for lipid accumulation and higher li-polysis rate than myotubes from severely obese donors without diabetes. This indicatesthat a difference in intramyocellular lipid turnover might be fundamental in evolving type2 diabetes.