Vis enkel innførsel

dc.contributor.authorPidoux, Guillaume
dc.contributor.authorWitczak, Oliwia
dc.contributor.authorJarnæss, Elisabeth
dc.contributor.authorMyrvold, Linda
dc.contributor.authorUrlaub, Henning
dc.contributor.authorStokka, Anne Jorunn
dc.contributor.authorKüntziger, Thomas Michel
dc.contributor.authorTasken, Kjetil
dc.date.accessioned2012-01-27T10:02:41Z
dc.date.available2012-04-07T02:02:36Z
dc.date.issued2011-10-07
dc.identifier.citationPidoux, G., Witczak, O., Jarnæss, E., Myrvold, L., Urlaub, H., Stokka, A.J. ... Tasken, Kjetil. (2011). Optic atrophy 1 is an A-kinase anchoring protein on lipid droplets that mediates adrenergic control of lipolysis. EMBO Journal, 30 (21)en_US
dc.identifier.issn0261-4189
dc.identifier.otherFRIDAID 846036
dc.identifier.urihttps://hdl.handle.net/10642/1077
dc.description.abstractAdrenergic stimulation of adipocytes yields a cAMP signal that activates protein kinase A (PKA). PKA phosphorylates perilipin, a protein localized on the surface of lipid droplets that serves as a gatekeeper to regulate access of lipases converting stored triglycerides to free fatty acids and glycerol in a phosphorylation-dependent manner. Here, we report a new function for optic atrophy 1 (OPA1), a protein known to regulate mitochondrial dynamics, as a dual-specificity A-kinase anchoring protein associated with lipid droplets. By a variety of protein interaction assays, immunoprecipitation and immunolocalization experiments, we show that OPA1 organizes a supramolecular complex containing both PKA and perilipin. Furthermore, by a combination of siRNA-mediated knockdown, reconstitution experiments using full-length OPA1 with or without the ability to bind PKA or truncated OPA1 fused to a lipid droplet targeting domain and cellular delivery of PKA anchoring disruptor peptides, we demonstrate that OPA1 targeting of PKA to lipid droplets is necessary for hormonal control of perilipin phosphorylation and lipolysis.en_US
dc.language.isoengen_US
dc.publisherNature Publishing Groupen_US
dc.relation.ispartofseriesEMBO Journal;30 (21)
dc.subjectMembranesen_US
dc.subjectLipolysisen_US
dc.subjectCell signalingen_US
dc.subjectcAMPen_US
dc.subjectPerilipinen_US
dc.subjectOPA1en_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk molekylærbiologi: 711en_US
dc.titleOptic atrophy 1 is an A-kinase anchoring protein on lipid droplets that mediates adrenergic control of lipolysisen_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US
dc.description.versionPostprint version of published article.en_US
dc.identifier.doihttp://dx.doi.org/10.1038/emboj.2011.365


Tilhørende fil(er)

Thumbnail
Thumbnail

Denne innførselen finnes i følgende samling(er)

Vis enkel innførsel