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dc.contributor.authorHaugen, Øyvind Pernell
dc.contributor.authorVallenari, Evan Michael
dc.contributor.authorBelhaj, Imen
dc.contributor.authorSmåstuen, Milada Cvancarova
dc.contributor.authorStorm-Mathisen, Jon
dc.contributor.authorBergersen, Linda Hildegard
dc.contributor.authorÅmellem, Ingrid
dc.date.accessioned2020-01-10T13:37:22Z
dc.date.accessioned2020-01-28T12:07:45Z
dc.date.available2020-01-10T13:37:22Z
dc.date.available2020-01-28T12:07:45Z
dc.date.issued2020-12-02
dc.identifier.citationHaugen, Vallenari, Belhaj, Småstuen, Storm-Mathisen, Bergersen, Åmellem. Blood lactate dynamics in awake and anaesthetized mice after intraperitoneal and subcutaneous injections of lactate—sex matters. PeerJ. 2020en
dc.identifier.issn2167-8359
dc.identifier.issn2167-8359
dc.identifier.urihttps://hdl.handle.net/10642/8016
dc.description.abstractLactate treatment has shown a therapeutic potential for several neurological diseases, including Alzheimer’s disease. In order to optimize the administration of lactate for studies in mouse models, we compared blood lactate dynamics after intraperitoneal (IP) and subcutaneous (SC) injections. We used the 5xFAD mouse model for familial Alzheimer’s disease and performed the experiments in both awake and anaesthetized mice. Blood glucose was used as an indication of the hepatic conversion of lactate. In awake mice, both injection routes resulted in high blood lactate levels, mimicking levels reached during high-intensity training. In anaesthetized mice, SC injections resulted in significantly lower lactate levels compared to IP injections. Interestingly, we observed that awake males had significantly higher lactate levels than awake females, while the opposite sex difference was observed during anaesthesia. We did not find any significant difference between transgenic and wild-type mice and therefore believe that our results can be generalized to other mouse models. These results should be considered when planning experiments using lactate treatment in mice.en
dc.description.sponsorshipFunding contribution was obtained from the Norwegian Health Association, project ID 14848, project leader Linda H. Bergersen.en
dc.language.isoenen
dc.publisherPeerJen
dc.relation.ispartofseriesPeerJ;8: e8328
dc.rightsThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectLactateen
dc.subjectGlucoseen
dc.subjectIsofluraneen
dc.subjectSex differencesen
dc.subjectIntraperitoneal injectionsen
dc.subjectSubcutaneous injectionsen
dc.titleBlood lactate dynamics in awake and anaesthetized mice after intraperitoneal and subcutaneous injections of lactate—sex mattersen
dc.typeJournal articleen
dc.typePeer revieweden
dc.date.updated2020-01-10T13:37:22Z
dc.description.versionpublishedVersionen
dc.identifier.doihttps://dx.doi.org/10.7717/peerj.8328
dc.identifier.cristin1766725
dc.source.journalPeerJ


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This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed.
Med mindre annet er angitt, så er denne innførselen lisensiert som This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed.