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dc.contributor.authorSmith, Nicole
dc.contributor.authorWoldemariam, Nardos Tesfaye
dc.contributor.authorRise, Matthew L.
dc.contributor.authorAndreassen, Rune
dc.date.accessioned2020-06-02T14:14:45Z
dc.date.accessioned2020-06-03T10:05:05Z
dc.date.available2020-06-02T14:14:45Z
dc.date.available2020-06-03T10:05:05Z
dc.date.issued2020-06-02
dc.identifier.citationSmith, N., Woldemariam, N.T., Rise, M.L. & Andreassen, R. (2020). Characterization of miRNAs in cultured atlantic salmon head kidney monocyte-like and macrophage-like cells. International Journal of Molecular Sciences, 21(11). doi:https://doi.org/10.3390/ijms21113989en
dc.identifier.issn1422-0067
dc.identifier.issn1422-0067
dc.identifier.urihttps://hdl.handle.net/10642/8680
dc.description.abstractMacrophages are among the first cells to respond to infection and disease. While microRNAs (miRNAs) are involved in the process of monocyte-to-macrophage differentiation in mammals, less is known in teleost fish. Here, Atlantic salmon head kidney leukocytes (HKLs) were used to study the expression of miRNAs in response to in vitro culture. The morphological analysis of cultures showed predominantly monocyte-like cells on Day 1 and macrophage-like cells on Day 5, suggesting that the HKLs had differentiated from monocytes to macrophages. Day 5 HKLs also contained a higher percentage of phagocytic cells. Small RNA sequencing and qPCR analysis were applied to examine the miRNA diversity and expression. There were 370 known mature Atlantic salmon miRNAs in HKLs. Twenty-two miRNAs (15 families) were downregulated while 44 miRNAs (25 families) were upregulated on Day 5 vs. Day 1. Mammalian orthologs of many of the differentially expressed (DE) miRNAs are known to regulate macrophage activation and differentiation, while the teleost-specific miR-2188, miR-462 and miR-731 were also DE and are associated with immune responses in fish. In silico predictions identified several putative target genes of qPCR-validated miRNAs associated with vertebrate macrophage differentiation. This study identified Atlantic salmon miRNAs likely to influence macrophage differentiation, providing important knowledge for future functional studies.en
dc.language.isoenen
dc.relation.ispartofseriesInternational Journal of Molecular Sciences;Volume 21 / Issue 11
dc.rights© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectAtlantic salmonen
dc.subjectMacrophagesen
dc.subjectmiRNAen
dc.subjectHead kidney culturesen
dc.subjectCell differentiationen
dc.subjectSequencingen
dc.titleCharacterization of miRNAs in cultured atlantic salmon head kidney monocyte-like and macrophage-like cellsen
dc.typeJournal articleen
dc.typePeer revieweden
dc.date.updated2020-06-02T14:14:45Z
dc.description.versionpublishedVersionen
dc.identifier.doihttps://dx.doi.org/10.3390/ijms21113989
dc.identifier.cristin1813533
dc.source.journalInternational Journal of Molecular Sciences
dc.relation.projectIDNorges forskningsråd: 280839
dc.relation.projectIDNorges forskningsråd: 254849
dc.relation.projectIDDirektoratet for internasjonalisering og kvalitetsutvikling i høgare utdanning: HPN-2015-10026


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© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Except where otherwise noted, this item's license is described as © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).