| dc.contributor.author | Nikolic, Natasa | |
| dc.contributor.author | Aas, Vigdis | |
| dc.date.accessioned | 2020-01-14T08:39:47Z | |
| dc.date.accessioned | 2020-03-27T09:49:19Z | |
| dc.date.available | 2020-01-14T08:39:47Z | |
| dc.date.available | 2020-03-27T09:49:19Z | |
| dc.date.issued | 2018-10-27 | |
| dc.identifier.citation | Nikolic N, Aas V. Electrical pulse stimulation of primary human skeletal muscle cells. Methods in molecular biology. 2019;1889:17-24 | en |
| dc.identifier.isbn | 978-1-4939-8896-9 | |
| dc.identifier.issn | 1064-3745 | |
| dc.identifier.issn | 1064-3745 | |
| dc.identifier.issn | 1940-6029 | |
| dc.identifier.uri | https://hdl.handle.net/10642/8321 | |
| dc.description.abstract | Electrical pulse stimulation (EPS) is an in vitro method of inducing contractions in cultured
skeletal muscle cells of human and animal origin. Motor neuron activation of muscle fibres
can be replaced by applying EPS on differentiated skeletal muscle cells (myotubes) in culture
[1,2].
Here we describe two protocols for EPS of human myotubes in 6-well plates: acute, highfrequency
(single bipolar pulses of 2 ms, 100 Hz for 200 ms every 5th sec for 5-60 min, 10-30 V) and chronic, low-frequency (single bipolar pulses of 2 ms, 1 Hz 10-30 V for 48 h) at the end of a 7 days long differentiation. | en |
| dc.language.iso | en | en |
| dc.publisher | Springer | en |
| dc.relation.ispartof | Myogenesis; Methods and Protocols | |
| dc.relation.ispartofseries | Methods in molecular biology;MIMB, volume 1889 | |
| dc.rights | This is a post-peer-review, pre-copyedit version of a chapter published in Myogenesis: Methods and Protocols, part of the Methods in Molecular Biology book series (MIMB, volume 1889). The final authenticated version is available online at: https://dx.doi.org/10.1007/978-1-4939-8897-6_2 | en |
| dc.subject | Skeletal muscles | en |
| dc.subject | Myotubes | en |
| dc.subject | Contractions | en |
| dc.subject | Electrical pulse stimulations | en |
| dc.title | Electrical pulse stimulation of primary human skeletal muscle cells | en |
| dc.type | Chapter | en |
| dc.type | Journal article | en |
| dc.type | Peer reviewed | en |
| dc.date.updated | 2020-01-14T08:39:47Z | |
| dc.description.version | acceptedVersion | en |
| dc.identifier.doi | https://dx.doi.org/10.1007/978-1-4939-8897-6_2 | |
| dc.identifier.cristin | 1772013 | |
| dc.source.journal | Methods in molecular biology | |
