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dc.contributor.authorRundblad, Amanda
dc.contributor.authorLarsen, Sunniva
dc.contributor.authorMyhrstad, Mari
dc.contributor.authorOttestad, Inger
dc.contributor.authorThoresen, Magne
dc.contributor.authorHolven, Kirsten Bjørklund
dc.contributor.authorUlven, Stine Marie
dc.date.accessioned2020-01-31T20:21:18Z
dc.date.accessioned2020-02-04T14:05:53Z
dc.date.available2020-01-31T20:21:18Z
dc.date.available2020-02-04T14:05:53Z
dc.date.issued2019-03-20
dc.identifier.citationRundblad A, Larsen S, Myhrstad MC, Ottestad I, Thoresen M, Holven KB, Ulven S. Differences in peripheral blood mononuclear cell gene expression and triglyceride composition in lipoprotein subclasses in plasma triglyceride responders and non-responders to omega-3 supplementation. Genes & Nutrition. 2019;14(10):1-13en
dc.identifier.issn1555-8932
dc.identifier.issn1555-8932
dc.identifier.issn1865-3499
dc.identifier.urihttps://hdl.handle.net/10642/8057
dc.description.abstractBackground:Intake of the marine omega-3 fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)reduces fasting triglyceride (TG) levels and may thereby lower cardiovascular disease risk. However, there are largeinter-individual differences in the TG-lowering effect of omega-3 supplementation. Genotype differences partly explainthis variation, but gene-environment interactions leading to gene expression differences may also be important. In thisstudy, we aimed to investigate baseline differences and differences in the change in peripheral blood mononuclear cell(PBMC) gene expression and lipoprotein subclass TG levels between TG responders and non-responders to omega-3fatty acid supplementation.Methods:In a previous randomized controlled trial, healthy normotriglyceridemic subjects (n= 35, 71% women)received 1.6 g EPA + DHA/day for 7 weeks. In this exploratory sub-study, we defined TG responders as subjects havinga TG reduction beyond the 20% day-to-day variation and non-responders as having a TG change between−20% and+ 20% after omega-3 supplementation. PBMC gene expression was measured using microarray, and lipoproteinsubclasses were measured using nuclear magnetic resonance spectroscopy.Results:Eight subjects were defined as responders with a median TG reduction of 37%, and 16 subjects were definedas non-responders with a median TG change of 0%. At baseline, responders had higher TG levels in two of four high-density lipoprotein (HDL) subclasses and 909 gene transcripts (p≤0.05) were differentially expressed compared to non-responders. During the intervention, the plasma TG reduction among responders was reflected in TG reductions in fourof six different very low-density lipoprotein subclasses and three of four different HDL subclasses. Compared to non-responders, the expression of 454 transcripts was differentially altered in responders (p≤0.05). Pathway analyses revealed that responders had altered signaling pathways related to development and immune function. In addition,two of the top 10 enriched pathways in responders compared to non-responders were related to lysophosphatidic acid signaling.Conclusion:TG responders and non-responders to omega-3 supplementation have different lipoprotein subclass andPBMC gene expression profiles at baseline and different lipoprotein subclass and PBMC gene expression responses toomega-3 supplementation. These gene expression differences may partially explain the variability in TG response observed after omega-3 supplementation.en
dc.description.sponsorshipThis study was funded by the University of Oslo, OsloMet – Oslo Metropolitan University, the research council of Norway, and the Throne-Holst Foundation for Nutrition Research, Oslo, Norway.en
dc.language.isoenen
dc.publisherBMC (part of Springer Nature)en
dc.relation.ispartofseriesGenes and Nutrition;14, Article number: 10 (2019)
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectEicosapentaenoic acidsen
dc.subjectDocosahexaenoic acidsen
dc.subjectOmega 3en
dc.subjectTranscriptomicsen
dc.subjectMicroarraysen
dc.subjectTriglyceridesen
dc.titleDifferences in peripheral blood mononuclear cell gene expression and triglyceride composition in lipoprotein subclasses in plasma triglyceride responders and non-responders to omega-3 supplementationen
dc.typeJournal articleen
dc.typePeer revieweden
dc.date.updated2020-01-31T20:21:18Z
dc.description.versionpublishedVersionen
dc.identifier.doihttps://dx.doi.org/10.1186/s12263-019-0633-y
dc.identifier.cristin1711922
dc.source.journalGenes & Nutrition


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This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
Except where otherwise noted, this item's license is described as This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.