Utilization of lactic acid in human myotubes and interplay with glucose and fatty acid metabolism
| dc.contributor.author | Lund, Jenny | |
| dc.contributor.author | Aas, Vigdis | |
| dc.contributor.author | Tingstad, Ragna Husby | |
| dc.contributor.author | Van Hees, Alfons | |
| dc.contributor.author | Nikolic, Natasa | |
| dc.date.accessioned | 2018-12-18T10:09:58Z | |
| dc.date.accessioned | 2019-01-08T15:14:46Z | |
| dc.date.available | 2018-12-18T10:09:58Z | |
| dc.date.available | 2019-01-08T15:14:46Z | |
| dc.date.issued | 2018-06-29 | |
| dc.identifier.citation | Lund J, Aas V, Tingstad R, Van Hees A, Nikolic N. Utilization of lactic acid in human myotubes and interplay with glucose and fatty acid metabolism. Scientific Reports. 2018;8 | en |
| dc.identifier.issn | 2045-2322 | |
| dc.identifier.issn | 2045-2322 | |
| dc.identifier.uri | https://hdl.handle.net/10642/6489 | |
| dc.description.abstract | Once assumed only to be a waste product of anaerobe glycolytic activity, lactate is now recognized as an energy source in skeletal muscles. While lactate metabolism has been extensively studied in vivo, underlying cellular processes are poorly described. This study aimed to examine lactate metabolism in cultured human myotubes and to investigate effects of lactate exposure on metabolism of oleic acid and glucose. Lactic acid, fatty acid and glucose metabolism were studied in myotubes using [14C(U)] lactic acid, [14C]oleic acid and [14C(U)]glucose, respectively. Myotubes expressed both the MCT1, MCT2, MCT3 and MCT4 lactate transporters, and lactic acid was found to be a substrate for both glycogen synthesis and lipid storage. Pyruvate and palmitic acid inhibited lactic acid oxidation, whilst glucose and α-cyano-4-hydroxycinnamic acid inhibited lactic acid uptake. Acute addition of lactic acid inhibited glucose and oleic acid oxidation, whereas oleic acid uptake was increased. Pretreatment with lactic acid for 24 h did not affect glucose or oleic acid metabolism. By replacing glucose with lactic acid during the whole culturing period, glucose uptake and oxidation were increased by 2.8-fold and 3-fold, respectively, and oleic acid oxidation was increased 1.4-fold. Thus, lactic acid has an important role in energy metabolism of human myotubes. | en |
| dc.description.sponsorship | This work was supported by grants from the University of Oslo and OsloMet – Oslo Metropolitan University. The sponsors did not have any involvement in study design, collection, analysis or interpretation of data, writing of the manuscript, or decision to submit the manuscript for publication. | en |
| dc.language.iso | en | en |
| dc.publisher | Nature Research | en |
| dc.relation.ispartofseries | Scientific Reports;8, Article number: 9814 (2018) | |
| dc.rights | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2018. | en |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | Lactic acids | en |
| dc.subject | Human myotubes | en |
| dc.subject | Glucose | en |
| dc.subject | Fatty acid metabolisms | en |
| dc.title | Utilization of lactic acid in human myotubes and interplay with glucose and fatty acid metabolism | en |
| dc.type | Journal article | en |
| dc.type | Peer reviewed | en |
| dc.date.updated | 2018-12-18T10:09:57Z | |
| dc.description.version | publishedVersion | en |
| dc.identifier.doi | http://dx.doi.org/10.1038/s41598-018-28249-5 | |
| dc.identifier.cristin | 1595191 | |
| dc.source.journal | Scientific Reports |
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Except where otherwise noted, this item's license is described as This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2018.