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dc.contributor.authorAlfsnes, Kristian
dc.contributor.authorFrye, Stephan Alfons
dc.contributor.authorEriksson, JE
dc.contributor.authorTonjum, Tone
dc.contributor.authorEldholm, Vegard
dc.contributor.authorBrynildsrud, Ola Brønstad
dc.contributor.authorBohlin, Jon
dc.contributor.authorHarrison, OB
dc.contributor.authorHood, D
dc.contributor.authorMaiden, Martin C.J.
dc.contributor.authorAmbur, Ole Herman
dc.date.accessioned2018-10-03T16:58:58Z
dc.date.accessioned2018-10-19T11:33:28Z
dc.date.available2018-10-03T16:58:58Z
dc.date.available2018-10-19T11:33:28Z
dc.date.issued2018-09-25
dc.identifier.citationAlfsnes k, Frye SA, Eriksson, Tonjum T, Eldholm V, Brynildsrud OB, Bohlin J, Harrison O, Hood D, Maiden MC, Ambur OH. A genomic view of experimental intra- and interspecies transformation of a rifampicin resistance allele into Neisseria meningitidis. Microbial Genomics. 2018en
dc.identifier.issn2057-5858
dc.identifier.issn2057-5858
dc.identifier.urihttps://hdl.handle.net/10642/6269
dc.description.abstractThe spread of antibiotic resistance within and between different bacterial populations is a major health problem on a global scale. The identification of genetic transformation in genomic data from Neisseria meningitidis, the meningococcus (Mc), and other bacteria is problematic, since similar or even identical alleles may be involved. A particular challenge in naturally transformable bacteria generally is to distinguish between common ancestry and true recombined sites in sampled genome sequences. Furthermore, the identification of recombination following experimental transformation of homologous alleles requires identifiable differences between donor and recipient, which in itself influences the propensity for homologous recombination (HR). This study identifies the distribution of HR events following intraspecies and interspecies Mc transformations of rpoB alleles encoding rifampicin resistance by whole-genome DNA sequencing and single nucleotide variant analysis. The HR events analysed were confined to the genomic region surrounding the single nucleotide genetic marker used for selection. An exponential length distribution of these recombined events was found, ranging from a few nucleotides to about 72 kb stretches. The lengths of imported sequences were on average found to be longer following experimental transformation of the recipient with genomic DNA from an intraspecies versus an interspecies donor (P<0.001). The recombination events were generally observed to be mosaic, with donor sequences interspersed with recipient sequence. Here, we present four models to explain these observations, by fragmentation of the transformed DNA, by interruptions of the recombination mechanism, by secondary recombination of endogenous self-DNA, or by repair/replication mechanisms.en
dc.description.sponsorshipNorges forskningsråd 261669 Norges forskningsråd 220901 Norges forskningsråd 271874 Norges forskningsråd 196394 Helse Sør-Øst RHF 2014050en
dc.language.isoenen
dc.publisherMicrobiology Societyen
dc.relation.ispartofseriesMicrobial Genomics;
dc.rights2018 The Authors This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.subjectExperimental evolutionsen
dc.subjectTransformationsen
dc.subjectHomologous recombinationsen
dc.subjectGenome sequencingsen
dc.titleA genomic view of experimental intra- and interspecies transformation of a rifampicin resistance allele into Neisseria meningitidisen
dc.typeJournal articleen
dc.typePeer revieweden
dc.date.updated2018-10-03T16:58:58Z
dc.description.versionpublishedVersionen
dc.identifier.doihttp://dx.doi.org/10.1099/mgen.0.000222
dc.identifier.cristin1617708
dc.source.journalMicrobial Genomics


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