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dc.contributor.authorDas, Mrinal Kumaren_US
dc.contributor.authorAndreassen, Runeen_US
dc.contributor.authorHaugen, Trine B.en_US
dc.contributor.authorFuru, Karien_US
dc.date.accessioned2016-02-09T13:38:25Z
dc.date.available2016-02-09T13:38:25Z
dc.date.issued2016en_US
dc.identifier.citationDas, M. K., Andreassen, R., Haugen, T. B., & Furu, K. (2016). Identification of Endogenous Controls for Use in miRNA Quantification in Human Cancer Cell Lines. Cancer Genomics & Proteomics, 13(1), 63-68.en_US
dc.identifier.issn1109-6535en_US
dc.identifier.otherFRIDAID 1319848en_US
dc.identifier.urihttps://hdl.handle.net/10642/3004
dc.description.abstractBackground: miRNAs play important roles in multiple biological processes, and deregulation has been linked to several human diseases, including cancer. Studying changes in miRNA expression in cancer development is commonly performed in vitro in human cancer cell lines using quantitative polymerase chain reaction (qPCR), a method requiring the use of a robust reference gene that displays stable expression across all samples. Materials and Methods: Using the NormFinder software, a selection of commonly used endogeneous controls and miRNAs were tested in six human cancer cell lines to identify for the most suitable gene for use as a reference. Results: The frequently used endogenous control U6B small nuclear RNA (RNU6B) was found to be an unsuitable reference for normalization. The most suitable single endogeneous control identified was miR-25-3p, whereas the best combination of two endogeneous controls was miR-25-3p and miR-93-5p. Conclusion: We identified a single and a pair of miRNAs suitable for use as endogenous controls when performing qPCR-based miRNA expression analyses in human cancer cell lines.en_US
dc.language.isoengen_US
dc.publisherInternational Institute of Anticancer Researchen_US
dc.relation.ispartofseriesCancer Genomics & Proteomics;13(1)en_US
dc.subjectmiRNAen_US
dc.subjectendogenous controlen_US
dc.subjectqPCRen_US
dc.subjecthuman cancer cell linesen_US
dc.subjectquantification of gene expressionen_US
dc.subjectVDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Onkologi: 762en_US
dc.titleIdentification of Endogenous Controls for Use in miRNA Quantification in Human Cancer Cell Linesen_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US


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