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dc.contributor.authorMyhrstad, Marien_US
dc.contributor.authorUlven, Stine Marieen_US
dc.contributor.authorGünther, Clara-Cecilieen_US
dc.contributor.authorOttestad, Ingeren_US
dc.contributor.authorHolden, Mariten_US
dc.contributor.authorRyeng, Einaren_US
dc.contributor.authorBorge, Grethe Iren Andersenen_US
dc.contributor.authorKohler, Achimen_US
dc.contributor.authorBrønner, Kirsti Wettreen_US
dc.contributor.authorThoresen, Magneen_US
dc.contributor.authorHolven, Kirsten Bjørklunden_US
dc.date.accessioned2015-02-26T11:38:06Z
dc.date.available2015-02-26T11:38:06Z
dc.date.issued2014en_US
dc.identifier.citationMyhrstad, M. C., Ulven, S. M., Günther, C. C., Ottestad, I., Holden, M., Ryeng, E., ... & Holven, K. B. (2014). Fish oil supplementation induces expression of genes related to cell cycle, endoplasmic reticulum stress and apoptosis in peripheral blood mononuclear cells: a transcriptomic approach. Journal of internal medicine, 276(5), 498-511.en_US
dc.identifier.issnOnline 1365-2796en_US
dc.identifier.issnPrint: 0954-6820en_US
dc.identifier.otherFRIDAID 1180802en_US
dc.identifier.urihttps://hdl.handle.net/10642/2404
dc.description.abstractBackground. Fish oil supplementation has been shown to alter gene expression of mononuclear cells both in vitro and in vivo. However, little is known about the total transcriptome profile in healthy subjects after intake of fish oil. We therefore investigated the gene expression profile in peripheral blood mononuclear cells (PBMCs) after intake of fish oil for 7 weeks using transcriptome analyses. Design. In a 7-week, double-blinded, randomized, controlled, parallel-group study, healthy subjects received 8 g day 1 fish oil (1.6 g day 1 eicosapentaenoic acid + docosahexaenoic acid) (n = 17) or 8 g day 1 high oleic sunflower oil (n = 19). Microarray analyses of RNA isolated from PBMCs were performed at baseline and after 7 weeks of intervention. Results. Cell cycle, DNA packaging and chromosome organization are biological processes found to be upregulated after intake of fish oil compared to high oleic sunflower oil using a moderated t-test. In addition, gene set enrichment analysis identified several enriched gene sets after intake of fish oil. The genes contributing to the significantly different gene sets in the subjects given fish oil compared with the control group are involved in cell cycle, endoplasmic reticulum (ER) stress and apoptosis. Gene transcripts with common motifs for 35 known transcription factors including E2F, TP53 and ATF4 were upregulated after intake of fish oil. Conclusion. We have shown that intake of fish oil for 7 weeks modulates gene expression in PBMCs of healthy subjects. The increased expression of genes related to cell cycle, ER stress and apoptosis suggests that intake of fish oil may modulate basic cellular processes involved in normal cellular function.en_US
dc.language.isoengen_US
dc.publisherThe Association for the Publication of the Journal of Internal Medicineen_US
dc.relation.ispartofseriesJournal of International Medicine;276(5)en_US
dc.subjectFish oilen_US
dc.subjectn-3 fatty aciden_US
dc.subjectPeripheral blood mononuclear cellsen_US
dc.subjectVDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Kardiologi: 771en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Cellebiologi: 471en_US
dc.titleFish oil supplementation induces Expression of genes related to cell cycle, endoplasmic reticulum stress and apoptosis in peripheral blood mononuclear cells: a transcriptomic approachen_US
dc.typeJournal articleen_US
dc.typePeer revieweden_US
dc.description.version© 2014 The Authors. Journal of Internal Medicine published by John Wiley & Sons Ltd on behalf of The Association for the Publication of the Journal of Internal Medicine. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.en_US
dc.identifier.doihttp://dx.doi.org/10.1111/joim.12217


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