dc.contributor.author | Guerriero, Ilaria | |
dc.contributor.author | Ramberg, Håkon Andre | |
dc.contributor.author | Sagini, Krizia | |
dc.contributor.author | Ramirez-Garrastacho, Manuel | |
dc.contributor.author | Tasken, Kristin Austlid | |
dc.contributor.author | Llorente, Alicia | |
dc.date.accessioned | 2022-03-18T07:46:46Z | |
dc.date.available | 2022-03-18T07:46:46Z | |
dc.date.created | 2021-07-09T14:01:06Z | |
dc.date.issued | 2021-06-30 | |
dc.identifier.citation | PLOS ONE. 2021, 16:e025828 (6), 1-11. | en_US |
dc.identifier.issn | 1932-6203 | |
dc.identifier.uri | https://hdl.handle.net/11250/2986016 | |
dc.description.abstract | The β2-adrenergic receptor has been shown to be involved in neuroendocrine differentiation and to contribute to the development of aggressive prostate cancer. In this study we have investigated whether miR-196a plays a role in the regulation of the β2-adrenergic receptor in the LNCaP prostate cancer cell line. Our results show that the expression of miR-196a is elevated in LNCaP prostate cancer cells with reduced levels of β2-adrenergic receptor after stably transfection with three different shRNAs. Furthermore, treatment with β-blockers showed that this upregulation is strictly related to the low levels of β2-adrenergic receptor and not to the inhibition of the receptor signaling activity. Finally, we found that the reduced ability of LNCaP cells with low levels of β2-adrenergic receptor to initiate neuroendocrine differentiation under androgen depletion conditions is mediated by miR-196a. In conclusion, this study provides the rational for a role of miR-196a in the β2-adrenergic receptor mediated neuroendocrine differentiation of LNCaP prostate cancer cells. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | Public Library of Science | en_US |
dc.relation.ispartofseries | PLOS ONE;16 (6): e0253828 | |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.subject | Prostate cancer | en_US |
dc.subject | MicroRNAs | en_US |
dc.subject | Transfections | en_US |
dc.subject | Metastasis | en_US |
dc.subject | Adrenergic signal transduction | en_US |
dc.subject | Neurites | en_US |
dc.title | Implication of β2-adrenergic receptor and miR-196a correlation in neurite outgrowth of LNCaP prostate cancer cells | en_US |
dc.type | Peer reviewed | en_US |
dc.type | Journal article | en_US |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | © 2021 Guerriero et al | en_US |
dc.source.articlenumber | e025828 | en_US |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.doi | https://doi.org/10.1371/journal.pone.0253828 | |
dc.identifier.cristin | 1921190 | |
dc.source.journal | PLOS ONE | en_US |
dc.source.volume | 16 | en_US |
dc.source.issue | 6 | en_US |
dc.source.pagenumber | 1-11 | en_US |