dc.contributor.author | Iglesias, Rosario | |
dc.contributor.author | Ferreras, José M | |
dc.contributor.author | Llorente, Alicia | |
dc.contributor.author | Citores, Lucía | |
dc.date.accessioned | 2022-03-10T13:51:38Z | |
dc.date.available | 2022-03-10T13:51:38Z | |
dc.date.created | 2021-07-07T13:57:41Z | |
dc.date.issued | 2021-01-30 | |
dc.identifier.citation | Toxins. 2021, 13 (2), 1-15. | en_US |
dc.identifier.issn | 2072-6651 | |
dc.identifier.uri | https://hdl.handle.net/11250/2984334 | |
dc.description.abstract | Ebulin l is an A-B toxin, and despite the presence of a B chain, this toxin displays much less toxicity to cells than the potent A-B toxin ricin. Here, we studied the binding, mechanisms of endocytosis, and intracellular pathway followed by ebulin l and compared it with ricin. COS-1 cells and HeLa cells with inducible synthesis of a mutant dynamin (K44A) were used in this study. The transport of these toxins was measured using radioactively or fluorescently labeled toxins. The data show that ebulin l binds to cells to a lesser extent than ricin. Moreover, the expression of mutant dynamin does not affect the endocytosis, degradation, or toxicity of ebulin l. However, the inhibition of clathrin-coated pit formation by acidification of the cytosol reduced ebulin l endocytosis but not
toxicity. Remarkably, unlike ricin, ebulin l is not transported through the Golgi apparatus to intoxicate the cells and ebulin l induces apoptosis as the predominant cell death mechanism. Therefore, after binding to cells, ebulin l is taken up by clathrin-dependent and -independent endocytosis into the endosomal/lysosomal system, but there is no apparent role for clathrin and dynamin in productive intracellular routing leading to intoxication. | en_US |
dc.description.sponsorship | This work was funded by grants BIO39/VA39/14 and BIO/VA17/15 (Consejería de Sanidad, Junta de Castilla y León) to L.C. and grant VA033G19 (Consejería de Educación, Junta de Castilla y León) to the GIR ProtIBio. A.L. acknowledges the South-Eastern Norway Regional Health Authority, The Norwegian Cancer Society, and The Norwegian Research Council. | en_US |
dc.language.iso | eng | en_US |
dc.publisher | MDPI | en_US |
dc.relation.ispartofseries | Toxins;Volume 13 / Issue 2 | |
dc.rights | Navngivelse 4.0 Internasjonal | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/deed.no | * |
dc.subject | Apoptosis | en_US |
dc.subject | Clathrin | en_US |
dc.subject | Dynamin | en_US |
dc.subject | Intracellular transport | en_US |
dc.subject | rRNA N-glycosylase | en_US |
dc.subject | Ribosome-inactivating proteins | en_US |
dc.title | Ebulin l Is Internalized in Cells by Both Clathrin-Dependent and -Independent Mechanisms and Does Not Require Clathrin or Dynamin for Intoxication | en_US |
dc.type | Peer reviewed | en_US |
dc.type | Journal article | en_US |
dc.description.version | publishedVersion | en_US |
dc.rights.holder | © 2021 by the author | en_US |
dc.source.articlenumber | 102 | en_US |
cristin.ispublished | true | |
cristin.fulltext | original | |
cristin.qualitycode | 1 | |
dc.identifier.doi | https://doi.org/10.3390/toxins13020102 | |
dc.identifier.cristin | 1920731 | |
dc.source.journal | Toxins | en_US |
dc.source.volume | 13 | en_US |
dc.source.issue | 2 | en_US |
dc.source.pagenumber | 1-15 | en_US |
dc.relation.project | Consejería de Sanidad, Junta de Castilla y León: BIO39/VA39/14 | en_US |
dc.relation.project | Consejería de Sanidad, Junta de Castilla y León: BIO/VA17/15 | en_US |
dc.relation.project | Consejería de Educación, Junta de Castilla y León: VA033G19 | en_US |