Whole genome sequencing and antibiotic diffusionassays, provide new insight on drug resistance in thegenus Pedobacter
dc.contributor.author | Ullmann, Ingvild Falkum | |
dc.contributor.author | Nygaard, Anders Benteson | |
dc.contributor.author | Tunsjø, Hege | |
dc.contributor.author | Charnock, Colin | |
dc.date.accessioned | 2020-06-02T10:00:30Z | |
dc.date.accessioned | 2020-06-04T10:04:35Z | |
dc.date.available | 2020-06-02T10:00:30Z | |
dc.date.available | 2020-06-04T10:04:35Z | |
dc.date.issued | 2020-05-09 | |
dc.identifier.citation | Ullmann IF, Nygaard A, Tunsjø HS, Charnock C. Whole genome sequencing and antibiotic diffusionassays, provide new insight on drug resistance in thegenus Pedobacter. FEMS Microbiology Ecology. 2020;96(6) | en |
dc.identifier.issn | 0168-6496 | |
dc.identifier.issn | 0168-6496 | |
dc.identifier.issn | 1574-6941 | |
dc.identifier.uri | https://hdl.handle.net/10642/8682 | |
dc.description.abstract | A total of four strains of the ‘environmental superbug’ Pedobacter isolated from sludge produced at Norwegian drinking water treatment plants, were characterized by whole genome sequencing and antibiotic susceptibility assays. As with previous studies on members of this genus, we found that the isolates were multi-drug resistant, and that this resistance included clinically important beta-lactams, aminoglycosides and the fluoroquinolone ciprofloxacin. Using the minION sequencing platform (Oxford Nanopore Technologies) combined with HiSeq PE150 Illumina sequencing data, the four isolates were assembled into genomes of single contigs. Analysis of the genomes revealed potential genetic factors possibly underlying some of the specific resistances observed. Metallo-beta-lactamase activity was detected in one isolate, and the same isolate contained a putative metallo-betalactamase gene resembling pedo-2. Furthermore, several genes related to multidrug efflux systems were found using the resistance database CARD. Additionally, the present study extends our knowledge on the phylogeny of this genus, adding four new genomes to the existing 50. | en |
dc.description.sponsorship | This project was funded by Oslo Metropolitan University, Norway. | en |
dc.language.iso | en | en |
dc.publisher | Oxford University Press (OUP) | en |
dc.relation.ispartofseries | FEMS Microbiology Ecology;Volume 96, Issue 6, June 2020 | |
dc.rights | This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. | en |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.subject | Antimicrobial resistance | en |
dc.subject | MinION sequencing | en |
dc.subject | Pedobacter spp | en |
dc.subject | Environmental microbiology | en |
dc.subject | Drinking water | en |
dc.title | Whole genome sequencing and antibiotic diffusionassays, provide new insight on drug resistance in thegenus Pedobacter | en |
dc.type | Journal article | en |
dc.type | Peer reviewed | en |
dc.date.updated | 2020-06-02T10:00:30Z | |
dc.description.version | publishedVersion | en |
dc.identifier.doi | https://dx.doi.org/10.1093/femsec/fiaa088 | |
dc.identifier.cristin | 1813471 | |
dc.source.journal | FEMS Microbiology Ecology |
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Med mindre annet er angitt, så er denne innførselen lisensiert som This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.