A preliminary study on the potential of Nanopore MinION and Illumina MiSeq 16S rRNA gene sequencing to characterize building-dust microbiomes
dc.contributor.author | Nygaard, Anders Benteson | |
dc.contributor.author | Tunsjø, Hege | |
dc.contributor.author | Meisal, Roger | |
dc.contributor.author | Charnock, Colin | |
dc.date.accessioned | 2020-03-10T08:17:06Z | |
dc.date.accessioned | 2020-03-20T22:53:02Z | |
dc.date.available | 2020-03-10T08:17:06Z | |
dc.date.available | 2020-03-20T22:53:02Z | |
dc.date.issued | 2020-01-30 | |
dc.identifier.citation | Nygaard A, Tunsjø HS, Meisal R, Charnock C. A preliminary study on the potential of Nanopore MinION and Illumina MiSeq 16S rRNA gene sequencing to characterize building-dust microbiomes. Scientific Reports. 2020;10(1):3209 | en |
dc.identifier.issn | 2045-2322 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | https://hdl.handle.net/10642/8293 | |
dc.description.abstract | There is a growing awareness of the importance of indoor microbiomes for human health. Given their complexity, these microbiomes can only be adequately surveyed using high throughput sequencing techniques. Oxford Nanopore's MinION is the newest third generation sequencing technology on the market. With its many advantages such as portability, user friendliness, simplicity, speed of sequencing and long read length, the technology is now an actual contender to established sequencing platforms. MinION's main disadvantage is a relatively low read accuracy compared to several other platforms, although this is constantly improving. The present study, which appears to be the first of its kind, provides the results of a preliminary analysis of the microbial communities in indoor environments based on 16S rRNA gene amplicon sequencing, using both the Oxford Nanopore Technologies (ONT) MinIOn and the Illumina MiSeq DNA sequencers. At the level of family and above, there was no significant difference between the microbial compositions as revealed by the two platforms. However, at the genus, and particularly at the species level, the ONT MinION reported greater taxonomic resolution than Illumina MiSeq. | en |
dc.language.iso | en | en |
dc.publisher | Nature Research (part of Springer Nature) | en |
dc.relation.ispartofseries | Scientific Reports;10, Article number: 3209 (2020) | |
dc.rights | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. | en |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.subject | Bacterial genes | en |
dc.subject | Data processing | en |
dc.subject | Environmental microbiology | en |
dc.subject | Dust microbiome building | en |
dc.title | A preliminary study on the potential of Nanopore MinION and Illumina MiSeq 16S rRNA gene sequencing to characterize building-dust microbiomes | en |
dc.type | Journal article | en |
dc.type | Peer reviewed | en |
dc.date.updated | 2020-03-10T08:17:05Z | |
dc.description.version | publishedVersion | en |
dc.identifier.doi | https://dx.doi.org/10.1038/s41598-020-59771-0 | |
dc.identifier.cristin | 1800783 | |
dc.source.journal | Scientific Reports |
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Med mindre annet er angitt, så er denne innførselen lisensiert som This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.