dc.contributor.author | Lagström, Sonja | |
dc.contributor.author | van der Weele, Pascal | |
dc.contributor.author | Rounge, Trine Ballestad | |
dc.contributor.author | Christiansen, Irene Kraus | |
dc.contributor.author | King, Audrey | |
dc.contributor.author | Ambur, Ole Herman | |
dc.date.accessioned | 2020-02-03T13:39:06Z | |
dc.date.accessioned | 2020-02-04T09:26:48Z | |
dc.date.available | 2020-02-03T13:39:06Z | |
dc.date.available | 2020-02-04T09:26:48Z | |
dc.date.issued | 2019-08-09 | |
dc.identifier.citation | Lagström SL, van der Weele, Rounge TB, Christiansen IK, King, Ambur OH. HPV16 whole genome minority variants in persistent infections from young Dutch women. Journal of Clinical Virology. 2019;119:24-30 | en |
dc.identifier.issn | 1386-6532 | |
dc.identifier.issn | 1386-6532 | |
dc.identifier.issn | 1873-5967 | |
dc.identifier.uri | https://hdl.handle.net/10642/8047 | |
dc.description.abstract | Background: Chronic infections by one of the oncogenic human papillomaviruses (HPVs) are responsible for near 5% of the global cancer burden and HPV16 is the type most often found in cancers. HPV genomes displayunexpected levels of variation when deep-sequenced. Minor nucleotide variations (MNVs) may reveal HPVgenomic instability and HPV-related carcinogenic transformation of host cells. Objectives: The objective of this study was to investigate HPV16 genome variation at the minor variant level onpersisting HPV16 cervical infections from a population of young Dutch women. Study design:15 HPV16 infections were sequenced using a whole-HPV genome deep sequencing protocol (TaME-seq). One infection was followed over a three-year period, eight were followed over a two-year period, threewere followed over a one-year period and three infections had a single sampling point. Results and conclusions: Using a 1% variant frequency cutoff, we find on average 48 MNVs per HPV16 genomeand 1717 MNVs in total when sequencing coverage was > 100 × . Wefind the transition mutation T > C to be the most common, in contrast to other studies detecting APOBEC-related C > T mutation profiles in pre-cancerous and cancer samples. Our results suggest that the relative mutagenic footprint of HPV16 genomes may differ between the infections in this study and transforming lesions. In addition, we identify a number of MNVs that have previously been associated with higher incidence of high-grade lesions (CIN3+) in a population study. These findings may provide a starting point for future studies exploring causality between emerging HPV minorgenomic variants and cancer development. | en |
dc.description.sponsorship | Ministry of Health, Welfare and Sports, the Netherlands and the South-Eastern Norway Regional Health Authority (Grant ID 2016020) are greatly acknowledged for funding. | en |
dc.language.iso | en | en |
dc.publisher | Elsevier | en |
dc.relation.ispartofseries | Journal of Clinical Virology;Volume 119, October 2019 | |
dc.rights | © 2019 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/). | en |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.subject | Human papillomaviruses | en |
dc.subject | Persistent infections | en |
dc.subject | Genome variations | en |
dc.subject | Mutational signatures | en |
dc.title | HPV16 whole genome minority variants in persistent infections from young Dutch women | en |
dc.type | Journal article | |
dc.type | Peer reviewed | en |
dc.date.updated | 2020-02-03T13:39:05Z | |
dc.description.version | publishedVersion | en |
dc.subject.hrcs | Kreft: Underbyggende Forskning | |
dc.subject.hrcs | Cancer : Underpinning Research | |
dc.identifier.doi | https://dx.doi.org/10.1016/j.jcv.2019.08.003 | |
dc.identifier.cristin | 1720463 | |
dc.source.journal | Journal of Clinical Virology | |
dc.subject.nsi | VDP::Matematikk og naturvitenskap: 400::Basale biofag: 470::Genetikk og genomikk: 474 | |
dc.subject.nsi | VDP::Mathematics and natural scienses: 400::Basic biosciences: 470::Genetics and genomics: 474 | |
dc.subject.nsi | VDP::Medisinske fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk mikrobiologi : 715 | |
dc.subject.nsi | VDP::Midical sciences: 700::Basic medical, dental and veterinary sciences: 710::Medical microbiology: 715 | |